Welqut Protease

Alternative Names: Not Available

WELQut Protease is a specific and recombinant serine protease derived from Staphylococcus aureus. It cleaves recombinant proteins with a recognition sequence consisting of the amino acid sequence Trp, Glu, Leu, Gln, and any amino acid (X). The protease cleaves externally from the recognition sequence, avoiding the addition of extra amino acids to the target protein. It is not affected by temperature (works between 4-30°C) or pH (pH 6.5-9.0), and does not require any specific buffers.

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  • Sku: PSB-enz-1113-100U
  • Vendor: ProSpec-Tany
$90.00 USD
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Title

Product Details

Product Description

Recombinant Staphylococcus aureus Welqut Protease is an enzyme that belongs to the serine protease family. This protease is derived from Staphylococcus aureus, a Gram-positive bacterium commonly found on the skin and mucous membranes of humans. With a molecular weight of approximately X kDa, this enzyme exhibits catalytic activity by cleaving peptide bonds in proteins. Its recombinant form allows for enhanced production and purification, making it a valuable tool in various biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 97 by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Welqut Protease contains 10 mM Na2HPO4, 50% glycerol, 1.8 mM KH2PO4, pH 7.3, 140 mM NaCl and 2.7 mM KCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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Welqut Protease

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