UPP1

Alternative Names: UDP, UPP, UPASE, UDRPASE

UPP1 enzyme catalyzes the reversible breakdown of uridine and deoxyuridine to form uracil and ribose- or deoxyribose-1-phosphate. These compounds are then utilized as sources of carbon and energy or in the production of pyrimidine bases for nucleotide synthesis. UPP1 belongs to the family of glycosyltransferases, more specifically known as pentosyltransferases. Pyrimidine nucleoside phosphorylases are enzymes that add ribose or deoxyribose to pyrimidine bases, resulting in the formation of nucleosides that can be incorporated into RNA or DNA.

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  • Sku: PSB-enz-258-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant E. coli Uridine phosphorylase is an enzyme that plays a significant role in nucleotide metabolism. Belonging to the family of phosphoribosyltransferases, this enzyme catalyzes the reversible phosphorolysis of uridine, yielding ribose-1-phosphate and uracil. With a molecular weight of approximately X kilodaltons, this enzyme's activity is crucial for the salvage pathway of pyrimidine nucleotide synthesis. Understanding the characteristics and functions of Recombinant E. coli Uridine phosphorylase is vital for various applications in biotechnology and pharmaceutical research.

Current Lead Time

7-14 Days

Product Specifications

Species
 E. Coli
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation UPP1 solution (1mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50mM NaCl and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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UPP1
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