Product Description

Uracil DNA Glycosylase (UDG) is an enzyme responsible for the removal of uracil residues from DNA molecules, thereby preventing the accumulation of potentially mutagenic lesions. Belonging to the family of DNA glycosylases, UDG plays a significant role in base excision repair pathways by specifically recognizing and excising uracil bases from DNA. With a molecular weight of approximately 25 kilodaltons, UDG efficiently maintains the integrity of DNA by catalyzing the hydrolysis of the N-glycosidic bond between uracil and the deoxyribose sugar, leaving behind an apyrimidinic site for further repair processes.

Current Lead Time

7-14 Days

Product Specifications

*For Research Use Only

Notes

Specific Activity: The Specific Activity was found to be 5U/µl Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C. UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. Inactiviation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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