UCHL5

Alternative Names: Ubiquitin Carboxyl-terminal Hydrolase L5, UCH-L5, Ubiquitin thioesterase L5, Ubiquitin Carboxyl-terminal Esterase L5, INO80 complex subunit R, INO80R, UCH37, CGI-70, EC 3.4.19.12

UCHL5 is a peptidase C12 family member that acts as a protease. It targets 'Lys-48'-linked polyubiquitin chains and is a deubiquitinating enzyme that is associated with the 19S regulatory subunit of the 26S proteasome.

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  • Sku: PSB-enz-097-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Ubiquitin Carboxyl-Terminal Esterase L5, a member of the ubiquitin-specific protease (USP) family, is an enzyme responsible for the removal of ubiquitin molecules from target proteins. With a molecular weight of approximately X kDa, this enzyme plays a significant role in the regulation of various cellular processes by deubiquitinating proteins. Recombinant Human Ubiquitin Carboxyl-Terminal Esterase L5's enzymatic activity contributes to the maintenance of protein homeostasis, cellular signaling, and protein degradation pathways. Its precise function and involvement in disease mechanisms are subjects of ongoing scientific investigation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at  pH 8.0, at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
39.7 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The UCHL5 protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 5mM DTT, 200mM NaCl, 0.1mM PMSF, 2mM EDTA and 30% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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UCHL5

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