UCHL3

Alternative Names: Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12.

Ubiquitin carboxyl-terminal hydrolase isozyme L3, a member of a gene family, functions to break down small C-terminal adducts of ubiquitin, producing the ubiquitin monomer. This enzyme, UCHL3, is involved in controlling neuronal development and spermatogenesis and is linked to neurodegenerative disorders. UCHL3 shares a 54% similarity with UCHL1.

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  • Sku: PSB-enz-057-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Ubiquitin Carboxyl-Terminal Esterase L3, also known as UCHL3, is a member of the ubiquitin carboxyl-terminal hydrolase family. This enzyme plays a vital role in the regulation of protein degradation pathways by cleaving ubiquitin molecules from protein substrates. With a molecular weight of approximately 27 kDa, UCHL3 exhibits deubiquitinating activity, contributing to the recycling of ubiquitin and maintaining cellular protein homeostasis. Its enzymatic function and involvement in various cellular processes make UCHL3 a valuable target for research in the field of protein degradation and related diseases.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C. Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
28.3 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The UCHL3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Storage Instruction

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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UCHL3

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