UBE2I

Alternative Names: EC 6.3.2.-, SUMO-conjugating enzyme UBC9, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.

Human Ubc9 is structurally and functionally similar to yeast Ubc9. However, instead of connecting with ubiquitin, it connects with a similar molecule called SUMO-1. The ubiquitin-dependent protein degradation system is responsible for breaking down abnormal and short-lived proteins. This system requires the activities of E1 and E2 enzymes for the conjugation of ubiquitin.

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  • Sku: PSB-enz-341-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Ubiquitin-Conjugating Enzyme E2I, a member of the E2 enzyme family, is a protein involved in the process of ubiquitination. Ubiquitination plays a critical role in protein degradation, cellular signaling, and DNA repair pathways. With a molecular weight of approximately X kilodaltons, this enzyme functions by transferring ubiquitin molecules to target proteins, marking them for subsequent degradation or altering their activity. Understanding the biochemical properties and functions of Recombinant Human Ubiquitin-Conjugating Enzyme E2I contributes to unraveling the intricate mechanisms underlying cellular processes and holds potential implications in various biological and medical research fields.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation UBE2I (1mg/ml) contains 50mM HEPES (pH7.4) 150mM NaCl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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UBE2I

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