UBE2F

Alternative Names: NEDD8-conjugating enzyme 2, NCE2, NEDD8-conjugating enzyme UBE2F, Ubiquitin-conjugating enzyme E2F (putative), NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, EC 6.3.2.-.

UBE2F is a member of the ubiquitin-conjugating enzyme family, specifically the UBE2F subfamily. It plays a crucial role in the ubiquitination process, which targets proteins for degradation via the proteasome. UBE2F receives the NEDD8 protein from the UBA3-NAE1 E1 complex and attaches it to other proteins. The RBX2-UBE2F complex neddylates specific target proteins, such as CUL5, as evidenced by its interaction with the E3 ubiquitin ligase RBX2, but not RBX1.

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  • Sku: PSB-enz-625-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human Ubiquitin-Conjugating Enzyme E2F is a member of the E2 family of ubiquitin-conjugating enzymes. It plays a crucial role in the ubiquitination process, transferring ubiquitin molecules to target proteins for degradation. With a molecular weight of approximately 20 kDa, this enzyme is essential for maintaining cellular homeostasis by regulating protein turnover and degradation pathways. Its precise function in the ubiquitin-proteasome system makes it a valuable tool for studying protein degradation mechanisms in various biological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The UBE2F solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 1mM DTT and 30% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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UBE2F
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