UBE2E1

Alternative Names: Ubiquitin carrier protein E1, Ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5), UBCH6, Ubiquitin-protein ligase E1, EC 6.3.2.19.

UBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family that accepts ubiquitin from the E1 complex and attaches it to other proteins. It specifically catalyzes the attachment of ISG15 to proteins, mediating the degradation of short-lived and abnormal proteins. In experimental settings, UBE2E1 is also capable of catalyzing 'Lys-48'-linked polyubiquitination.

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  • Sku: PSB-enz-647-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Ubiquitin Conjugating Enzyme E2E1 is a member of the E2 family of enzymes involved in the ubiquitination pathway. This enzyme plays a key role in the transfer of ubiquitin from E1 to specific protein substrates, marking them for degradation by the proteasome. Recombinant Human Ubiquitin Conjugating Enzyme E2E1 has a molecular weight of approximately 22 kDa, making it a crucial component in the regulation of protein turnover within the cell.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The UBE2E1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDDDSR ASTSSSSSSS SNQQTEKETN TPKKKESKVS MSKNSKLLST SAKRIQKELA DITLDPPPNC SAGPKGDNIY EWRSTILGPP GSVYEGGVFF LDITFTPEYP FKPPKVTFRT RIYHCNINSQ GVICLDILKD NWSPALTISK VLLSICSLLT DCNPADPLVG SIATQYMTNR AEHDRMARQW TKRYAT

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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UBE2E1

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