TPI1 Active

Alternative Names: Triosephosphate Isomerase 1, TIM, TPI.

TPI1 is a member of the triosephosphate isomerase family and it plays a crucial role in the conversion of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) during glycolysis and gluconeogenesis. Mutations in TPI1 result in triosephosphate isomerase deficiency (TPI deficiency), which is an autosomal recessive disorder. TPI deficiency is the most severe clinical disorder associated with glycolysis and is characterized by a variety of symptoms including neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy, and increased susceptibility to infection.

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  • Sku: PSB-enz-1013-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Triosephosphate Isomerase 1, Active is a protein enzyme that plays a significant role in the glycolysis pathway, specifically in the interconversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Belonging to the family of isomerases, this enzyme facilitates the conversion of triosephosphate isomers, contributing to the overall energy production and metabolism within cells. With a molecular weight of approximately X kDa, this recombinant protein exhibits catalytic activity and is commonly employed in biochemical research and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 28.8 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation TPI1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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TPI1 Active
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