tPA Sf9

Alternative Names: EC 3.4.21.68, Tissue-type plasminogen activator, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT

Tissue plasminogen activator, also known as PLAT or tPA, is a serine protease that converts the inactive enzyme plasminogen into the active enzyme plasmin. Plasmin is responsible for breaking down fibrin, a protein involved in blood clot formation. PLAT also plays a role in cell migration and tissue remodeling. If PLAT activity is increased, it can lead to excessive bleeding. Conversely, if PLAT activity is decreased, it can cause blood clots to form.

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  • Sku: PSB-enz-1011-1µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Tissue Plasminogen Activator, Sf9, is a bioengineered protein used in medical applications. Belonging to the serine protease family, this protein plays a significant role in the breakdown of blood clots. With a molecular weight of approximately X kDa, it is produced using the Spodoptera frugiperda (Sf9) insect cell expression system. This recombinant protein holds potential in thrombolytic therapy, aiding in the dissolution of blood clots and restoring blood flow.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
61.3 kDa
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation tPA protein solution (0.25mg/ml) containing 50mM MES buffer(pH 5.5 ), 40% glycerol, 5mM CaCl2, 1mM DTT and 0.5M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

QEIHARFRRG ARSYQVICRD EKTQMIYQQH QSWLRPVLRS NRVEYCWCNS GRAQCHSVPV KSCSEPRCFN GGTCQQALYF SDFVCQCPEG FAGKCCEIDT RATCYEDQGI SYRGTWSTAE SGAECTNWNS SALAQKPYSG RRPDAIRLGL GNHNYCRNPD RDSKPWCYVF KAGKYSSEFC STPACSEGNS DCYFGNGSAY RGTHSLTESG ASCLPWNSMI LIGKVYTAQN PSAQALGLGK HNYCRNPDGD AKPWCHVLKN RRLTWEYCDV PSCSTCGLRQ YSQPQFRIKG GLFADIASHP WQAAIFAKHR RSPGERFLCG GILISSCWIL SAAHCFQERF PPHHLTVILG RTYRVVPGEE EQKFEVEKYI VHKEFDDDTY DNDIALLQLK SDSSRCAQES SVVRTVCLPP ADLQLPDWTE CELSGYGKHE ALSPFYSERL KEAHVRLYPS SRCTSQHLLN RTVTDNMLCA GDTRSGGPQA NLHDACQGDS GGPLVCLNDG RMTLVGIISW GLGCGQKDVP GVYTKVTNYL DWIRDNMRPHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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tPA Sf9

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