Thrombin - Human

Alternative Names: Not Available

Thrombin is a vital enzyme in blood clotting. It converts fibrinogen into fibrin strands, which are insoluble and help form clots. Thrombin also plays a role in several coagulation-related reactions by activating various factors. Additionally, it activates platelets by binding to receptors on their surface. Due to its specific proteolytic capabilities, thrombin is widely used in biochemistry. In particular, the cleavage site of thrombin is commonly employed to remove purification tags from recombinant fusion proteins after purification. This cleavage site is highly specific, targeting the Arginine-Glycine bond.

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  • Sku: PSB-pro-339-0.5mg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Human Thrombin is a serine protease that plays a vital role in the coagulation cascade, specifically in the conversion of fibrinogen to fibrin during blood clot formation. Belonging to the family of enzymes known as serine proteases, human thrombin is a critical component of the hemostatic system. With a molecular weight of approximately 37 kilodaltons, this enzyme is involved in various physiological processes, including wound healing and maintaining hemostasis. Understanding the characteristics and functions of human thrombin is crucial for comprehending the intricate mechanisms underlying blood clotting.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Human Plasma
Purity
Activity
The specific activity was found to be 2435 NIH Units/mg.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The Factor-IIa contains 0.15M NaCl, 0.1M Tris, pH 7.4.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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Thrombin - Human

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