TDG

Alternative Names: Thymine-DNA Glycosylase, EC 3.2.2.29, G/T Mismatch-Specific Thymine DNA Glycosylase

Thymine-DNA glycosylase (TDG) belongs to the TDG/mug DNA glycosylase family and is a nuclear protein. TDG corrects G/T mismatches to G/C pairs by breaking the carbon-nitrogen bond between the DNA sugar-phosphate backbone and the mispaired thymin. It also removes uracil and 5-bromouracil from mispairings with guanine. The TDG enzyme plays a crucial role in protecting cells from genetic mutations caused by the spontaneous deamination of 5-methylcytosine and cytosine.

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  • Sku: PSB-enz-649-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Thymine-DNA Glycosylase is an enzyme that belongs to the DNA glycosylase/AP lyase family. It plays a critical role in the base excision repair pathway by recognizing and removing thymine from mismatched DNA. This enzyme has a molecular weight of approximately 25 kDa and is essential for maintaining genomic stability by repairing DNA damage caused by environmental factors or endogenous processes. Its precise function and mechanism of action make it a valuable tool in molecular biology research and therapeutic applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The TDG solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 0.4M Urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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TDG

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