TARS, Sf9

Alternative Names: Threonyl-tRNA synthetase cytoplasmic, Threonine-tRNA ligase, ThrRS, EC 6.1.1.3, MGC9344, PL-7, TARS.

Threonyl-tRNA Synthetase is an 83 kDa protein that belongs to the aminoacyl-tRNA synthetase family. These enzymes play a vital role in protein biosynthesis by attaching amino acids to tRNA molecules. Threonyl-tRNA Synthetase is specifically recognized by PL-7 antibodies, which are found in some patients with polymyositis and dermatomyositis. Early findings indicate that the presence of PL-7 antibodies, similar to Jo-1 antibodies, may be associated with a higher risk of lung involvement. However, further research involving a larger sample size is needed to confirm this.

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  • Sku: PSB-enz-304-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Threonyl t-RNA Synthetase, Sf9, is a protein that belongs to the aminoacyl-tRNA synthetase family. This enzyme plays a vital role in protein synthesis by catalyzing the attachment of threonine to its cognate tRNA molecule. With a molecular weight of approximately X kDa, this recombinant protein is commonly used in research and biotechnological applications to study the mechanisms underlying tRNA aminoacylation. Its significance lies in its ability to accurately and efficiently incorporate threonine into the growing polypeptide chain during translation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Insect cells
Purity 95 by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM NaCl, and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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TARS, Sf9

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