Product Description

Recombinant Taq Plus DNA Polymerase is a thermostable enzyme that belongs to the DNA polymerase I family. With a molecular weight of approximately 94 kDa, this polymerase is widely utilized in molecular biology applications for its robust DNA amplification capabilities. It exhibits 5'→3' polymerase activity and possesses a 5'→3' exonuclease activity, enabling efficient DNA synthesis and proofreading functions. Recombinant Taq Plus DNA Polymerase is an invaluable tool in various PCR-based techniques, offering reliable and accurate amplification of DNA templates for research and diagnostic purposes.

Current Lead Time

7-14 Days

Product Specifications

*For Research Use Only

Notes

10x Reaction Buffer: 200 mM TrisHCI (pH 8.8) 100 mM KCI 100 mM (NH4)2SO4 20 mM Mg SO4 1% Triton X-100 1 mg/ml bovine serum albumin (BSA). Reaction Conditions: DNA synthesis is performed in 100?l of mixture containing 20-200μm dNTPs, 0.3-1μm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O. 2. 10 x reaction buffer. 3. dNTPs. 4. DNA template and primers. 5. Taq Plus.

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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