T5 Exonuclease

Alternative Names: T5 Exonuclease,

T5 Exonuclease

Recombinant T5 Exonuclease is an enzyme derived from the bacteriophage T5, belonging to the family of exonucleases. With a molecular weight of approximately X kDa, this enzyme plays a significant role in DNA replication and repair processes. Its ability to degrade single-stranded DNA in the 3' to 5' direction makes it a valuable tool in molecular biology research, particularly for applications such as DNA sequencing, site-directed mutagenesis, and removal of unwanted DNA fragments. Recombinant T5 Exonuclease offers precise and efficient DNA digestion, making it an indispensable component in various genetic engineering and biotechnology applications.

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  • Sku: PSB-enz-1184-5,000U
  • Vendor: ProSpec-Tany
$200.00 USD
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Title

Product Details

Product Description

Recombinant T5 Exonuclease is an enzyme derived from the bacteriophage T5, belonging to the family of exonucleases. With a molecular weight of approximately X kDa, this enzyme plays a significant role in DNA replication and repair processes. Its ability to degrade single-stranded DNA in the 3' to 5' direction makes it a valuable tool in molecular biology research, particularly for applications such as DNA sequencing, site-directed mutagenesis, and removal of unwanted DNA fragments. Recombinant T5 Exonuclease offers precise and efficient DNA digestion, making it an indispensable component in various genetic engineering and biotechnology applications.

Current Lead Time

7-14 Days

Product Specifications

Species
enzyme
Expression System E. coli
Purity 95 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation 10U/ul, 50mM Tris-HCl (25℃, pH 7.5), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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T5 Exonuclease

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