T4 DNA
Alternative Names: EC 6.5.1.1, DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4
- Sku: PSB-enz-286-20,000IU
- Vendor: ProSpec-Tany
Contact us directly for international purchases.
Product Description
Recombinant T4 DNA Ligase is an enzyme widely used in molecular biology for its ability to join DNA fragments together. Belonging to the DNA ligase family, this enzyme plays a vital role in the formation of phosphodiester bonds between adjacent DNA strands. With a molecular weight of approximately 68 kDa, Recombinant T4 DNA Ligase efficiently catalyzes the ligation of DNA fragments, making it a valuable tool in various genetic engineering applications. Its precise and reliable function makes it an indispensable asset in molecular biology research and DNA manipulation techniques.
Current Lead Time
7-14 Days
Product Specifications
|
Species |
T4 |
| Expression System | E. coli |
| Purity | 99.9 by SDS-PAGE |
|
Activity |
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.• T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.• Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50µM.• To dilute T4 DNA Ligase that will subsequently be stored at –20°C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used. |
| Activity Assay | |
| Animal Component Free (ACF) | Yes |
|
Molecular Weight |
N/A |
| Structure | Homodimer |
|
Endotoxin Concentration |
Not Available |
| Purification Method | Not Available |
|
Form |
Frozen |
| Formulation | 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µm in 5´ termini). Optimal ligation occurs at 16C. |
*For Research Use Only
If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation.
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T4 DNA