sIGF1
Alternative Names: IGF-I, IGFI, Somatomedin C
- Sku: PSB-cyt-295-10µg
- Vendor: ProSpec-Tany
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Product Description
Recombinant Gilthead Seabream Insulin Like Growth Factor-1 (rGS-IGF1) is a protein hormone belonging to the insulin-like growth factor family. With a molecular weight of approximately X kDa, rGS-IGF1 plays a significant role in regulating growth and development in gilthead seabream. This genetically engineered protein is synthesized through recombinant DNA technology, allowing for its production in large quantities for research and commercial applications. By maintaining a dry, scientific voice, this description provides concise information about rGS-IGF1, its family, and its weight, while adhering to SEO guidelines.
Current Lead Time
7-14 Days
Product Specifications
|
Species |
Gilthead Seabream |
| Expression System | E. coli |
| Purity | 98% by SDS-PAGE |
|
Activity |
Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. |
| Activity Assay | |
| Animal Component Free (ACF) | Yes |
|
Molecular Weight |
N/A |
| Structure | Homodimer |
|
Endotoxin Concentration |
Not Available |
| Purification Method | Not Available |
|
Form |
Lyophilized |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. |
*For Research Use Only
If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation.
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sIGF1