SAR1A

Alternative Names: SAR1, SAR1A, SAR1a, SARA, SARA1, GTP-binding protein SAR1a, COPII-associated small GTPase.

SAR1A is a key member of the small GTPase superfamily, playing a crucial role in the export of cargo from the Endoplasmic Reticulum (ER). This protein serves as a molecular switch, controlling interactions between proteins and lipids that determine the formation of COPII vesicle coats for ER cargo export. SAR1A interacts with a membrane-bound exchange factor called Sec12 when it is in its GDP-bound state, exchanging its GDP for GTP. Additionally, SAR1A is involved in transporting materials from the ER to the Golgi apparatus. It is necessary for maintaining the localization of SEC16A, a protein found on the ER membrane, by preventing its dissociation. The assembly of SEC16A on the ER membrane by SAR1A-GTP forms a structured scaffold that defines endoplasmic reticulum exit sites.

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  • Sku: PSB-pro-709-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human GTP-Binding Protein SAR1A is a protein involved in the regulation of vesicular trafficking and protein transport within cells. Belonging to the small GTPase superfamily, SAR1A plays a crucial role in the formation of COPII-coated vesicles, which facilitate the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. With a molecular weight of approximately 20 kDa, this protein is essential for maintaining proper cellular function and intracellular protein trafficking pathways. Its significance lies in its ability to regulate the secretion and sorting of various cargo molecules, making it a key player in cellular homeostasis.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 24.5 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The SAR1A solution contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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SAR1A
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