S100b His

Alternative Names: S100, S100B, S100beta, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, Protein S100-B, NEF.

S100b is a member of the S100 protein family that primarily exists as dimers. These proteins are involved in various cellular processes and are found in the cytoplasm or nucleus of many different cell types. S100b specifically is expressed in cells of the central nervous system, glial cells, and certain peripheral cells. It is located on chromosome 21q22.3, while the other S100 genes are clustered on chromosome 1q21. Determining the levels of S100b in serum can help monitor brain injury and melanoma. S100b proteins play roles in neurite extension, melanoma cell proliferation, calcium flux stimulation, PKC-mediated phosphorylation inhibition, astrocytosis and axonal proliferation, and microtubule assembly inhibition. Altered expression of the S100b gene and chromosomal rearrangements are associated with several neurological, neoplastic, and other diseases like Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

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  • Sku: PSB-pro-306-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Product Details

Product Description

Recombinant Human S100 Calcium Binding Protein B, His Tag is a protein derived from the S100 family, which belongs to the EF-hand superfamily. This protein plays a significant role in various cellular processes, including calcium signaling, cell proliferation, and differentiation. With a molecular weight of approximately X kDa, it exhibits calcium-dependent binding to target proteins, thereby modulating their functions. The His tag attached to this recombinant protein allows for easy purification and detection, enhancing its utility in research and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 12.8 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation S100B (1mg/ml) is supplied in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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S100b His
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