RPRD1B

Alternative Names: Not Available

Regulation of nuclear pre-mRNA domain-containing protein 1B (RPRD1B) interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of POLR2A, participating in CTD dephosphorylation. RPRD1B enhances RNA polymerase II binding to the CCDN1 promoter and the termination region prior to the poly-A site, but decreases binding after the poly-A site. It inhibits RNA polymerase II from reading through the 3' end termination site, potentially leading to a chromatin loop formation and drawing it back to the promoter. RPRD1B also boosts transcription of various cell cycle-related genes (CDK2, CDK4, CDK6, cyclin-E), while not affecting CDKN1A, CDKN1B, or cyclin-A expression. Additionally, RPRD1B promotes cell proliferation.

  •
  • Sku: PSB-pro-2205-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Regulation Of Nuclear Pre-MRNA Domain Containing 1B (RPRD1B) is a protein belonging to the RPRD1 family. This protein plays a significant role in the regulation of pre-mRNA processing in the nucleus. With a molecular weight of [insert weight], RPRD1B interacts with various components of the RNA polymerase II complex, facilitating the proper assembly and function of the transcription machinery. Its involvement in the regulation of gene expression highlights its importance in cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 39.3 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation RPRD1B protein solution (1mg/ml) in Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSFSES ALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEF TREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLK RTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKI TDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVT QVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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RPRD1B
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