RPL35A

Alternative Names: Ribosomal Protein L35a, 60S Ribosomal Protein L35a, Cell Growth-Inhibiting Gene 33 Protein, DBA5, L35A.

Ribosomes, responsible for protein synthesis, consist of a 40S subunit and a 60S subunit. These subunits are made up of 4 RNA species and around 80 structurally distinct proteins. RPL35A, a member of the L35AE family, is a component of the 60S subunit and is found in the cytoplasm. This protein binds to both initiator and elongator tRNAs, positioning itself at the P site or both the P and A sites of the ribosome. While originally thought to be located on chromosome 18, RPL35A has been confirmed to be situated at 3q29-qter. There are various transcript variants of this gene that use different transcription initiation sites and polyA signals. It is common for genes encoding ribosomal proteins to have multiple processed pseudogenes scattered throughout the genome.

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  • Sku: PSB-pro-1703-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Ribosomal Protein L35A is a protein encoded by the RPL35A gene in humans. Belonging to the ribosomal protein L35A family, it plays a significant role in the assembly and functioning of the ribosome, the cellular machinery responsible for protein synthesis. With a molecular weight of approximately X kDa, this protein is involved in stabilizing the interaction between the ribosomal subunits and facilitating the accurate positioning of the transfer RNA (tRNA) during translation. Understanding the structural and functional aspects of Recombinant Human Ribosomal Protein L35A contributes to our knowledge of ribosome biogenesis and its implications in various cellular processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 14.9 kDa
Structure Contact for Details
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The RPL35A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSGRLWS KAIFAGYKRG LRNQREHTAL LKIEGVYARD ETEFYLGKRC AYVYKAKNNT VTPGGKPNKT RVIWGKVTRA HGNSGMVRAK FRSNLPAKAI GHRIRVMLYP SRI

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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RPL35A
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