rMCP1

Alternative Names: Small inducible cytokine A2, SCYA2, CCL2, chemokine (C-C motif) ligand 2, MCP1, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, Immediate-early serum-responsive JE protein, HC11, Monocyte chemotactic and activating factor, MCAF, GDCF-2, GDCF-2 HC11, SMC-CF, HSMCR30, MGC9434.

CCL2 is a small cytokine belonging to the CC chemokine family that is also known as MCP-1. It plays a role in recruiting immune cells, such as monocytes, to sites of tissue injury and infection. CCL2 is expressed in the bone by mature osteoclasts and osteoblasts under the control of NF?B. It is produced as a protein precursor with a signal peptide of 23 amino acids and a mature peptide of 76 amino acids. CCL2 is a monomeric polypeptide with a molecular weight of approximately 13kDa. In humans, CCL2 is located on chromosome 17 and binds to cell surface receptors CCR2 and CCR5.

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  • Sku: PSB-chm-315-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2) is a chemokine belonging to the CC subfamily. With a molecular weight of approximately 13 kDa, CCL2 plays a role in the recruitment and activation of monocytes, T cells, and dendritic cells during inflammatory responses. This recombinant protein is commonly used in research settings to study the mechanisms of chemotaxis and immune cell migration.

Current Lead Time

7-14 Days

Product Specifications

Species
 Rat
Expression System E. coli
Purity 98% by SDS-PAGE
Activity
 ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay. ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Lyophilized
Formulation The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.

Storage Instruction

Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution CCL2 should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available
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rMCP1
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