rLDHA

Alternative Names: Not Available

LDHA, found mainly in muscle tissue, plays a key role in anaerobic glycolysis by converting L-lactate and NAD to pyruvate and NADH. Mutations in LDHA are associated with exertional myoglobinuria. LDH1 levels are reduced in essential thrombocythemia. LDHA is induced by estrogen through a non-genomic pathway. Decreased LDH-A activity leads to increased mitochondrial respiration and decreased mitochondrial membrane potential.

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  • Sku: PSB-enz-960-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Rat Lactate Dehydrogenase A is an enzyme belonging to the dehydrogenase family, specifically the lactate dehydrogenase subfamily. With a molecular weight of approximately 35 kDa, this enzyme plays a key role in the conversion of lactate to pyruvate during anaerobic glycolysis. Recombinant Rat Lactate Dehydrogenase A is commonly used in biochemical research and drug development due to its ability to catalyze this important metabolic reaction.

Current Lead Time

7-14 Days

Product Specifications

Species
Rat
Expression System Sf9 - Baculovirus cells
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
37.5 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation LDHA protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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rLDHA

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