RHOG

Alternative Names: Ras homolog family member G (rho G), rho-related GTP-binding protein RhoG, RhoG, ARHG, MGC125836, MGC125835.

RHOG is a member of the Rac subfamily of small G proteins and plays a crucial role in various intracellular signaling pathways. It is involved in the formation of membrane ruffles during macropinocytosis and is necessary for cell migration, particularly in the formation of cup-like structures during the trans-endothelial migration of leukocytes. Additionally, RHOG is involved in cell motility, gene transcription, endocytosis, neurite outgrowth, protection from cell detachment-induced apoptosis (called anoikis), and regulation of the neutrophil NADPH oxidase.

  •
  • Sku: PSB-pro-1136-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human Ras Homolog Gene Family Member G, also known as RhoG, is a protein belonging to the Ras homolog gene family. This gene family encodes small GTPases that play significant roles in various cellular processes, including cytoskeletal organization, cell migration, and cell adhesion. RhoG has a molecular weight of approximately 21 kilodaltons (kDa) and is involved in regulating actin dynamics and cell polarity. Its activation is mediated by guanine nucleotide exchange factors (GEFs) and is tightly regulated to ensure proper cellular functions. Understanding the functions and mechanisms of RhoG can provide valuable insights into various physiological and pathological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The RHOG solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.5M NaCl, 50mM Imidazole and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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RHOG
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