RBM17

Alternative Names: RNA-Binding Motif Protein 17, RNA Binding Motif Protein 17, RBM17, SPF45, Splicing Factor 45, Splicing Factor 45kDa, 45 kDa-splicing factor.

RBM17 is an RNA binding motif protein that plays a role in alternative splicing and the utilization of cryptic splice sites. It specifically binds to the single stranded 3'AG at the border between exons and introns, promoting its usage in the second catalytic step. RBM17 also stimulates the usage of a cryptic splice site caused by the beta-110 mutation in the HBB gene, leading to the development of sickle cell anemia.

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  • Sku: PSB-pro-1793-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human RNA Binding Motif Protein 17, commonly referred to as RBM17, is a protein that belongs to the RNA binding motif (RBM) family. RBM17 plays a significant role in RNA metabolism and post-transcriptional regulation. With a molecular weight of approximately X kilodaltons, RBM17 exhibits a conserved RNA recognition motif (RRM) structure, enabling it to interact with various RNA molecules. This protein's involvement in RNA processing and its potential implications in cellular functions make it a subject of interest in molecular biology and biomedical research.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Activity Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
47.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation RBM17 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V.

Storage Instruction

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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RBM17

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