RAD1

Alternative Names: Cell cycle checkpoint protein RAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, HRAD1, hRAD1, RAD-1, REC1, EC=3.1.11.2.

RAD1 is a component of the 9-1-1 complex, a cell cycle checkpoint complex that halts cell cycle progression in response to DNA damage or incomplete DNA replication. This complex consists of RAD1, Rad9, and Hus1, and is actively involved in the cellular response to DNA damage. RAD1 interacts with Rad17 and various components of the PCNA-loading heteropentamer known as replication factor C (RFC). In the case of DNA damage, RAD1 is recruited to the damaged site by the RAD17-RFC clamp loader complex. RAD1 acts as a platform on DNA for several proteins involved in a specific type of DNA repair called long-patch base excision repair (LP-BER). RAD1 also enhances the activity of DNA polymerase beta (POLB) by increasing its affinity for the 3''-OH end of the primer-template and stabilizing it at the sites where LP-BER occurs.

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  • Sku: PSB-pro-868-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human RAD1 is a protein that belongs to the DNA repair protein family. With a molecular weight of approximately X kDa, it plays a crucial role in maintaining genomic stability by participating in the nucleotide excision repair pathway. This protein is involved in the recognition and removal of damaged DNA, ensuring accurate DNA replication and transcription. Its significance lies in its ability to identify and repair various types of DNA damage, contributing to the overall integrity of the genetic material.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Heterotrimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation RAD1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.2M NaCl & 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQSEGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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RAD1
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