PURB

Alternative Names: PURB, PURBETA, Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B.

PURB is a single-stranded DNA-binding protein involved in transporting certain mRNAs in dendrites. In myoblasts and fibroblasts, PURB acts as a repressor in controlling the transcription of the alpha-actin gene in vascular smooth muscle. It is known to bind specifically to the single strand of a purine-rich element called PUR. This element is found in replication origins and gene flanking regions across a range of eukaryotes, including yeasts and humans.

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  • Sku: PSB-pro-1969-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Purine-Rich Element Binding Protein B (PURB) is a highly conserved RNA-binding protein that plays a significant role in post-transcriptional gene regulation. Belonging to the purine-rich element binding protein (PUR) family, PURB is involved in various cellular processes, including mRNA stability, translation, and splicing. With a molecular weight of approximately X kDa, PURB interacts with specific RNA sequences to modulate gene expression, thereby contributing to the intricate regulatory network governing cellular functions. Understanding the functional properties and mechanisms of PURB can provide valuable insights into the intricate molecular processes underlying gene regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The PURB solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PURB

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