PTTG1

Alternative Names: Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1, Esp1-associated protein, Securin, PTTG1, EAP1, PTTG, TUTR1, MGC126883, MGC138276.

PTTG1, or Securin, plays a key role in cell division by regulating the separation of sister chromatids. It is involved in chromosome stability, the p53/TP53 pathway, and DNA repair. PTTG1 has two additional roles: facilitating the transport of separase to the nucleus and inhibiting its catalytic activity. It is ubiquitinated by the APC and degraded by the Proteasome, which releases separase. During mitosis, PTTG1 blocks Separase/ESPL1 function to prevent the cleavage of the cohesin complex and the subsequent segregation of chromosomes. However, PTTG1 is also required to activate ESPL1. At the beginning of anaphase, PTTG1 is ubiquitinated, leading to its destruction and the liberation of ESPL1. PTTG1 contains two PXXP motifs necessary for its transforming and tumorigenic activities, as well as its stimulation of basic fibroblast growth factor expression. It also contains a D box essential for its degradation by the APC. The acidic C-terminal region of Securin can serve as a transactivation domain. Although primarily cytosolic, PTTG1 is partially localized in the nucleus. It is highly expressed in various tumors and exhibits transforming and tumorigenic activities in vitro and in vivo. Additionally, PTTG1 negatively regulates the transcriptional and apoptotic activity of TP53.

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  • Sku: PSB-pro-717-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human Pituitary Tumor-Transforming Protein 1 (PTTG1) is a protein belonging to the pituitary tumor-transforming gene family. With a molecular weight of approximately [insert weight], PTTG1 plays a significant role in regulating cell division and proliferation. As a transcription factor, it modulates the expression of various genes involved in cell cycle progression. PTTG1's involvement in tumorigenesis and its potential as a therapeutic target make it a subject of extensive scientific research.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 24.1 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The PTTG1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 20% glycerol, 1mM EDTA and 0.1mM PMSF.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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PTTG1
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