Protein-A G

Alternative Names: Not Available

The recombinant Protein A/G fusion protein is a combination of the IgG binding domains from Protein A and Protein G. It has four Fc binding domains from Protein A and two from Protein G, resulting in a total mass of 50.4 kDa. Protein A/G has a lower pH binding dependency compared to Protein A, but it possesses the combined properties of both Protein A and G. It is capable of binding to all subclasses of human IgG, making it useful for purifying polyclonal or monoclonal IgG antibodies when their subclasses are unknown. Additionally, Protein A/G binds to IgA, IgE, IgM, and IgD. When it comes to mouse IgG, Protein A/G can bind to all subclasses except for mouse IgA, IgM, and serum albumin. This makes it suitable for purifying and detecting mouse monoclonal IgG antibodies without interference from IgA, IgM, and serum albumin. Furthermore, chimeric Protein A/G exhibits a stronger affinity toward mouse monoclonal antibodies compared to Protein A or Protein G alone. It has also been utilized in the purification of macaque IgG.

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  • Sku: PSB-pro-646-1mg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Protein A/G is a genetically engineered fusion protein widely used in biotechnology and research applications. Belonging to the immunoglobulin-binding protein family, this protein is derived from the cell walls of certain strains of Staphylococcus aureus bacteria. With a molecular weight of approximately 50 kDa, Recombinant Protein A/G exhibits high affinity for both immunoglobulin G (IgG) and immunoglobulin A (IgA) from various species. Its unique ability to bind to multiple classes and subclasses of antibodies makes it a valuable tool in antibody purification, immunoprecipitation, and protein-protein interaction studies.

Current Lead Time

7-14 Days

Product Specifications

Species
 Protein
Expression System E. coli
Purity 97.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation Lyophilized white Powder containing no additives.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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Protein-A G
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