Protease
Alternative Names: Not Available
- Sku: PSB-enz-354-50IU
- Vendor: ProSpec-Tany
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Product Description
Recombinant Protease is a genetically engineered enzyme that plays a significant role in various biological processes. Belonging to the serine protease family, this protein is primarily involved in catalyzing the hydrolysis of peptide bonds. With a molecular weight ranging from several kilodaltons to several hundred kilodaltons, Recombinant Protease exhibits remarkable specificity towards its target substrates. Its precise and controlled action makes it a valuable tool in numerous scientific applications, including protein purification, biotechnology research, and pharmaceutical development.
Current Lead Time
7-14 Days
Product Specifications
Species |
enzyme |
Expression System | E. coli |
Purity | |
Activity |
Activity Not Available |
Activity Assay | |
Animal Component Free (ACF) | Yes |
Molecular Weight |
N/A |
Structure | Homodimer |
Endotoxin Concentration |
Not Available |
Purification Method | Not Available |
Form |
Frozen |
Formulation | Contact for Formulation |
*For Research Use Only
NotesCleavage Buffer: 50mM Tris-HCl, pH-7.0 (at 25°C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5°C prior to use
Cleavage Conditions: For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.
Unit Definition: One unit will cleave ?90% of 100 µg of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25°C) at 5°C for 16 hours.
If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation.
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Protease