PRDX6

Alternative Names: Peroxiredoxin-6, Antioxidant protein 2, 1-Cys peroxiredoxin, Acidic calcium-independent phospholipase A2, Non-selenium glutathione peroxidase, 24 kDa protein, Liver 2D page spot 40, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, NSGPx, PRDX6, AOP2, KIAA0106, PRX, p29, 1-Cys, MGC46173.

Peroxiredoxin 6 (PRDX6) is a thiol-specific antioxidant protein involved in redox regulation of the cell. It has two active sites and can reduce various types of hydroperoxides. PRDX6 may play a role in phospholipid turnover and protection against oxidative injury. It also alleviates oxidative stress and TGF-beta-induced abnormalities in human trabecular meshwork cells, and is necessary for blood vessel integrity in injured skin. At acidic pH, PRDX6 binds to reduced phospholipids, while at cytosolic pH it binds to oxidized phospholipids, indicating its role in repairing peroxidized cell membranes. Hyperoxidation of PRDX6 by hydrogen peroxide leads to cell cycle arrest at the G2/M transition through iPLA2 up-regulation. Increased expression of PRDX6 is associated with oligodendroglioma.

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  • Sku: PSB-enz-432-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Peroxiredoxin-6, belonging to the peroxiredoxin family, is a protein with a molecular weight of approximately X kDa. It plays a significant role in cellular antioxidant defense mechanisms by reducing hydrogen peroxide and organic hydroperoxides. This protein is involved in various physiological processes, including cell signaling, inflammation regulation, and protection against oxidative stress. Recombinant Human Peroxiredoxin-6 is widely studied for its potential therapeutic applications in conditions associated with oxidative damage and inflammatory responses.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 The specific activity was found to be approximately 95-120 pmole/min/µg. The enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The Peroxiredoxin-6 solution contains 20mM Tris-HCl buffer (pH8.0) and 20% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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PRDX6
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