PPP3R1

Alternative Names: Calcineurin B type I (19kDa), CNB1, CALNB1, Protein phosphatase 3 regulatory subunit B alpha, Protein phosphatase 3 (formerly 2B) regulatory subunit B (19kD) alpha isoform.

PPP3R1 is a key protein phosphatase involved in T cell activation. Calcineurin, composed of CnA and CnB subunits, dephosphorylates NF-AT, leading to its activation and gene expression in T cells.

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  • Sku: PSB-enz-066-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Protein Phosphatase 3, Regulatory subunit, Alpha Isozyme is a member of the protein phosphatase family, specifically the regulatory subunit alpha isozyme. This protein plays a role in regulating cellular processes by dephosphorylating target proteins. With a molecular weight of approximately 55 kDa, this enzyme is involved in various signaling pathways within the cell, making it a valuable tool for research and drug development in the field of molecular biology.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
21.5 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation PPP3R1 Human solution (1mg/1ml) containing 20mM Trsi HCL pH-8, 0.1M NaCl, 2mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PPP3R1

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