POU5F1

Alternative Names: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding protein 3, Oct-3, Octamer-binding transcription factor 3, OTF-3, Octamer-binding protein 4, Oct-4, OCT3, Octamer-binding transcription factor 4, OTF4, MGC22487.

POU5F1 is a homeodomain transcription factor that plays a significant role in maintaining self-renewal and pluripotency in embryonic stem (ES) and embryonic carcinoma (EC) cells. It has two DNA binding domains that can independently bind to specific DNA motifs. The flexibility of POU5F1 allows it to form heterodimers with other transcription factors or homodimers in different conformations, enabling it to bind to distinct DNA motifs. Human POU5F1 consists of a POUS domain and a POUH domain connected by a linker region. It specifically interacts with the octamer motif ATGCAAAT and requires two proline-rich domains for transactivation. POU5F1 works collaboratively with other transcription factors, such as Sox2 and Nanog, to regulate target genes and maintain stem cell potency and self-renewal.

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  • Sku: PSB-pro-088-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human POU Class 5 Homeobox 1, also known as POU5F1, is a protein belonging to the POU (Pit-Oct-Unc) family of transcription factors. This protein plays a critical role in maintaining pluripotency and self-renewal of embryonic stem cells. With a molecular weight of approximately 45 kDa, POU5F1 regulates the expression of genes involved in embryonic development and cellular differentiation. Understanding the functions and mechanisms of POU5F1 is of great interest in the field of stem cell research and regenerative medicine.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 32.2 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The POU5F1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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POU5F1
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