POLR3F

Alternative Names: DNA-directed RNA polymerase III subunit F, POLR3F, RNA polymerase III subunit C6, DNA-directed RNA polymerase III subunit RPC6, RPC6, RNA polymerase III 39 kDa subunit, RPC39

POLR3F is a member of the eukaryotic RPC34/RPC39 RNA polymerase subunit family, acting as a DNA-dependent RNA polymerase that transcribes DNA into RNA using ribonucleoside triphosphates as substrates. It is a subunit of eukaryotic RNA polymerase III, which transcribes 5S ribosomal RNA and tRNA genes. The POLR3F protein interacts with TFIIIB90 and TBP, two subunits of the RNA polymerase III transcription initiation factor IIIB.

  •
  • Sku: PSB-enz-650-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Polymerase III Polypeptide F is a member of the DNA polymerase family, specifically belonging to the polymerase III subfamily. With a molecular weight of approximately 130 kDa, this enzyme plays a critical role in DNA replication and repair processes. Recombinant Human Polymerase III Polypeptide F is commonly used in molecular biology research and biotechnology applications for its high fidelity and processivity during DNA synthesis.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 38.1 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The POLR3F solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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POLR3F
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