POLR2J

Alternative Names: DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 13.3 kDa subunit, POLR2J, POLR2J1, RPB11, RPB11a, RPB11m, hRPB14.

Polymerase (RNA) II Polypeptide J (POLR2J) is a subunit of RNA polymerase II, responsible for synthesizing messenger RNA in eukaryotes. It forms a core subassembly unit of the polymerase as a heterodimer with another subunit. POLR2J is widely expressed and highly expressed in the heart and skeletal muscle.

  •
  • Sku: PSB-pro-1260-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J is an enzyme that plays a vital role in transcription, specifically in the elongation phase. Belonging to the family of RNA polymerases, it is responsible for catalyzing the synthesis of RNA from a DNA template during gene expression. With a molecular weight of approximately 220 kDa, this enzyme facilitates the accurate and efficient transcription of genetic information. Its significance lies in its ability to maintain the integrity and fidelity of the transcription process, ensuring the accurate transfer of genetic information from DNA to RNA.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 15.7 kDa
Structure Heterodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation POLR2J protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

POLR2J
SUBHEADING

Recently viewed products