POLR2C

Alternative Names: RPB3, RPB31, RPB33, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit C, POLR2C, hsRPB3

Polymerase (RNA) II (DNA directed) Polypeptide C (POLR2C) is a significant subunit of RNA polymerase II. It is the third largest subunit and produces a cysteine rich area. POLR2C forms a core subassembly unit along with another polymerase subunit, POLR2J, in the form of a heterodimer.

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  • Sku: PSB-pro-1994-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide C, also known as POLR2C, is a crucial enzyme involved in the transcription process of DNA to RNA. Belonging to the polymerase family, POLR2C plays a significant role in the assembly and stability of RNA polymerase II, the enzyme responsible for transcribing protein-coding genes. With a molecular weight of approximately 31 kilodaltons, POLR2C acts as a non-catalytic subunit within the RNA polymerase II complex, contributing to its overall functionality. This Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide C is an important component in understanding the intricate mechanisms of gene expression and transcription regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
33.8 kDa
Structure Heterodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation POLR2C protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol 1mM DTT and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPYANQP TVRITELTDE NVKFIIENTD LAVANSIRRV FIAEVPIIAI DWVQIDANSS VLHDEFIAHR LGLIPLISDD IVDKLQYSRD CTCEEFCPEC SVEFTLDVRC NEDQTRHVTS RDLISNSPRV IPVTSRNRDN DPNDYVEQDD ILIVKLRKGQ ELRLRAYAKK GFGKEHAKWN PTAGVAFEYD PDNALRHTVY PKPEEWPKSE YSELDEDESQ APYDPNGKPE RFYYNVESCG SLRPETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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POLR2C

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