PF4 His

Alternative Names: PF-4, PF4, CXCL4, Iroplact, Oncostatin-A, SCYB4, MGC138298.

Platelet factor-4 is a 70-amino acid protein released from activated platelets that binds strongly to heparin. Its main role is to neutralize heparin-like molecules on blood vessel surfaces, inhibiting antithrombin III activity and promoting coagulation. PF4 also acts as a chemoattractant for neutrophils and fibroblasts, potentially impacting inflammation and wound healing. Oncostatin-A, part of the CXC chemokine family, is also mentioned. Human PF4 is used for diagnosing heparin-induced thrombocytopenia and as an angiogenesis inhibitor in cancer therapy.

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  • Sku: PSB-chm-015-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Platelet Factor-4 (CXCL4), His Tag is a member of the CXC chemokine family, known for its role in inflammation and immune response regulation. With a molecular weight of approximately 7.8 kDa, this protein is commonly used in research settings to study its interactions with other molecules and its effects on cellular processes. The addition of a Histidine (His) tag allows for easy purification and detection of the protein in experimental assays.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation PF4 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE S.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PF4 His

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