PEF1

Alternative Names: Peflin, PEF1, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1A, ABP32.

PEF1 is a penta-EF-hand protein that is closely related to the apoptosis associated gene ALG-2. It forms heterodimers with PDCD6/ALG2 and separates from PDCD6/ALG2 when calcium ions are present. In addition, PEF1 proteins are capable of binding calcium and are involved in multiple calcium-dependent activities in vertebrates.

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  • Sku: PSB-pro-099-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Penta-EF-Hand Domain Containing 1 is a protein that belongs to the EF-hand superfamily. With a molecular weight of approximately X kDa, it plays a significant role in calcium binding and signal transduction processes within cells. This protein is involved in regulating various cellular functions, including muscle contraction, enzyme activity, and gene expression. Understanding the structural and functional properties of Recombinant Human Penta-EF-Hand Domain Containing 1 is crucial for unraveling its physiological implications in health and disease.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
34.5 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The PEF1 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol and 0.2M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASY PYRQGCPGAA GQAPGAPPGSYYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGAAPGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALVNCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYTELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAVQGNIRLSFED FVTMTASRML.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PEF1

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