PCBD1

Alternative Names: PHS, PCD, PCBD, DCOH

PCBD1 enzyme plays a crucial role in phenylalanine hydroxylation and tetrahydrobiopterin biosynthesis, as well as controlling the homodimerization of HNF1. PCBD1 deficiency leads to hyperphenylalaninemia, while also preventing the formation of 7-pterins and promoting the formation of quinonoid-BH2. Additionally, PCBD1 serves as a coactivator for HNF1A-dependent transcription.

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  • Sku: PSB-enz-552-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Pterin-4-Alpha-Carbinolamine Dehydratase is an enzyme that belongs to the pterin-4-alpha-carbinolamine dehydratase family. This enzyme plays a key role in the biosynthesis of biopterin, which is essential for the production of neurotransmitters and nitric oxide. Recombinant Human Pterin-4-Alpha-Carbinolamine Dehydratase has a molecular weight of approximately 30 kDa and is commonly used in biochemical research and drug development.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
14.1 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The PCBD1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL DHHPEWFNVYNKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PCBD1

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