PAPSS1

Alternative Names: ATPSK1, SK 1, PAPSS 1, PAPS synthase 1, 3'-phosphoadenosine 5'-phosphosulfate synthase 1, 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1, Sulfurylase kinase 1, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, EC 2.7.1.25.

PAPSS1 is an enzyme involved in the sulfate activation pathway, producing 3'-phosphoadenylylsulfate (PAPS). It also plays a role in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

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  • Sku: PSB-enz-236-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 is an enzyme belonging to the sulfotransferase family, responsible for catalyzing the biosynthesis of 3'-phosphoadenosine 5'-phosphosulfate (PAPS). This enzyme plays a crucial role in the sulfation of various molecules, including hormones, neurotransmitters, and xenobiotics. With a molecular weight of approximately 35 kDa, Recombinant Human 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 is a key player in the regulation of sulfate metabolism and cellular signaling pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
70.9 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The PAPSS1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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PAPSS1

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