P4HB Active

Alternative Names: ERBA2L, PDIA1, PDI, PHD, PO4DB, PO4HB, P4Hbeta

P4HB is a common enzyme with multiple functions in the protein disulfide isomerase family. It forms a tetramer with two alpha and two beta subunits, playing a key role in hydroxylation of prolyl residues in preprocollagen. P4HB serves as a disulfide isomerase, utilizing its two thioredoxin domains to catalyze the formation, breakage, and rearrangement of disulfide bonds.

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  • Sku: PSB-enz-991-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Prolyl 4-Hydroxylase Beta, Active is an enzyme belonging to the prolyl hydroxylase family, specifically the beta subfamily. This enzyme plays a key role in the post-translational modification of proteins by catalyzing the hydroxylation of proline residues. With a molecular weight of approximately 60 kDa, Recombinant Human Prolyl 4-Hydroxylase Beta, Active is commonly used in research and biotechnology applications for its ability to modify collagen and other proteins.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT. Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
57.5 kDa
Structure Tetramer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The P4HB 1mg/ml protein solution contains 20mM Tris-HCl pH-8, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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P4HB Active

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