NUDT14

Alternative Names: UGPP, UGPPase, Uridine diphosphate glucose pyrophosphatase, UDPG pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14, NUDT14.

NUDT14, a member of the nudix hydrolase family, is a cytoplasmic protein that serves as a sugar donor in glycosylation reactions leading to glycogen production. It acts by hydrolyzing ADP-ribose into ribose 5-phosphate and AMP, as well as UDP-glucose into glucose 1-phosphate and UMP. NUDT14 functions as a homodimer, binding magnesium as a cofactor, and is encoded by a gene on human chromosome 14.

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  • Sku: PSB-enz-691-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Nudix Type Motif 14 is a protein belonging to the Nudix hydrolase family, which is involved in the hydrolysis of nucleoside diphosphate linked to another moiety. It has a molecular weight of approximately 14 kDa. This protein plays a role in maintaining cellular homeostasis by catalyzing the hydrolysis of potentially harmful nucleotide derivatives. Studies have shown that Recombinant Human Nudix Type Motif 14 may be implicated in various cellular processes, making it a subject of interest in the field of molecular biology.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
26.5 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The NUDT14 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NUDT14

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