NQO1, Active

Alternative Names: NAD(P)H dehydrogenase (quinone) 1, NAD(P)H:quinone oxidoreductase 1, Quinone reductase 1, QR1, DT-diaphorase, DTD, Azoreductase, Phylloquinone reductase, Menadione reductase, NQO1, DIA4, NMOR1, DHQU, NMORI.

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family, functioning as a cytoplasmic 2-electron reductase that plays a crucial role in cellular antioxidant defense by detoxifying quinones and preventing the formation of reactive oxygen species. It is involved in conjugation reactions of hydroquinones for detoxification pathways and biosynthetic processes like the gamma-carboxylation of glutamate residues in prothrombin synthesis. Changes in NQO1 expression are observed in tumors and Alzheimer’s disease, while mutations in the NQO1 gene are associated with tardive dyskinesia, increased hematotoxicity after exposure to benzene, and susceptibility to cancer.

  •
  • Sku: PSB-enz-1107-1µg
  • Vendor: ProSpec-Tany
$60.00 USD
Shipping calculated at checkout.

Contact us directly for international purchases.

Title
Report Product Listing Issue (e.g. Missing Data, or Questions)
Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human NAD(P)H Dehydrogenase Quinone 1, Active is a member of the NAD(P)H dehydrogenase quinone family, which plays a key role in the electron transport chain. This enzyme is responsible for catalyzing the oxidation of NADH and NADPH to generate a proton gradient across the mitochondrial membrane. With a molecular weight of approximately 75 kDa, Recombinant Human NAD(P)H Dehydrogenase Quinone 1, Active is a crucial component in cellular respiration and energy production.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Specific activity is > 1,000 pmol/min/ug. One unit will convert 1 pmoles resazurin to resorufin per minute at pH 7.5 at 25°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The NQO1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0),1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVESDLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIFQFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQGIHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETPLYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
View full details

NQO1, Active
SUBHEADING

Recently viewed products