NNMT Active

Alternative Names:

Nicotinamide N-methyltransferase, NNMT, EC 2.1.1.1.

NNMT is a methyltransferase enzyme primarily found in the liver, with lower levels present in other tissues such as the kidney, lung, skeletal muscle, placenta, and heart. It plays a role in the methylation of nicotinamide and other pyridines, which is important for drug metabolism. NNMT uses S-adenosyl methionine as a methyl donor. Studies have shown that NNMT expression is linked to tumor stage and disease-free survival time in hepatocellular carcinoma patients, making it a potential tumor marker for various cancers. Additionally, NNMT serum levels may be valuable for early detection and management of colorectal cancer.

  •
  • Sku: PSB-enz-1060-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human Nicotinamide N-Methyltransferase, Active is a member of the N-methyltransferase family, responsible for catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to nicotinamide. This enzyme plays a key role in the regulation of cellular metabolism and energy production. With a molecular weight of approximately 29 kDa, Recombinant Human Nicotinamide N-Methyltransferase, Active is commonly used in research studies to investigate its physiological functions and potential therapeutic applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
 Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 37.7 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation NNMT protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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NNMT Active
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