NMM

Alternative Names: NMM, Non-Muscle Myosin-II Regulatory Light Chain

Non-muscle myosins play a crucial role in cytokinesis, the process in which the cytoplasm of daughter cells is divided. They are also involved in cytoplasmic movements in tissues and the activation of motile cells like fibroblasts and macrophages. The activation of non-muscle myosin-II is brought about by phosphorylation of Ser33 in the KKRPQRATSN motif. This phosphorylation is carried out by a specific light chain kinase (MLCK) that is regulated by Ca+2 Calmodulin.

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  • Sku: PSB-pro-366-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Non-Muscle Myosin-II Regulatory Light Chain is a protein that plays a significant role in regulating non-muscle cell contraction and motility. Belonging to the myosin family, this protein is involved in various cellular processes, including cell division, migration, and adhesion. With a weight of approximately 20 kDa, this regulatory light chain modulates the activity of myosin-II motor proteins, thereby influencing cellular contractility. Its precise functioning and interactions contribute to the intricate machinery governing cell behavior and tissue organization.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 by SDS-PAGE
Activity
Assayed for phosphorilation by MLCK.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation NMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD.

Storage Instruction

Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NMM

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