NIT2

Alternative Names: Nitrilase homolog 2, Nitrilase family member 2, omega-amidase NIT2, Nit protein 2, MGC111199, EC 3.5.1.3.

NIT2 belongs to the nitrilase superfamily and exhibits omega-amidase activity. This activity helps in converting potentially toxic intermediates, alpha-ketoglutaramate and alpha-ketosuccinamate, into useful metabolites: alpha-ketoglutarate and oxaloacetate. NIT2 is found in various organisms and is believed to play a role in tumor suppression.

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  • Sku: PSB-enz-039-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Nitrilase Family Member 2 is a protein belonging to the nitrilase family, which plays a role in the hydrolysis of nitrile compounds. This enzyme has a molecular weight of approximately 40 kDa and is involved in the detoxification of cyanide and the metabolism of plant hormones. Recombinant Human Nitrilase Family Member 2 is a promising candidate for biotechnological applications due to its catalytic properties and potential industrial uses in the pharmaceutical and agricultural sectors.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The NIT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 0.1M NaCl, and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NIT2

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