NDUFAF2

Alternative Names: Mimitin mitochondrial, Myc-induced mitochondrial protein, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, NDUFA12-like protein, NDUFAF2, NDUFA12L, B17.2-like, B17.2L, mimitin.

Mimitin, also known as NDUFAF2, is a crucial component of the NDUFA12 subunit family within complex I of the mitochondria. It serves as the "entry enzyme" for oxidative phosphorylation, aiding in the transfer of electrons from NADH to coenzyme Q. Additionally, Mimitin acts as a molecular chaperone to assist in the assembly of mitochondrial complex I.

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  • Sku: PSB-enz-150-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human NADH Dehydrogenase 1 Alpha Subcomplex, Assembly Factor 2 is a protein involved in the assembly of complex I of the mitochondrial respiratory chain. Belonging to the NDUFAF family, it plays a critical role in the proper functioning of the electron transport chain. With a molecular weight of approximately 27 kDa, this protein is essential for the assembly and stability of complex I, which is crucial for cellular respiration and energy production.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation NDUFAF2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 200mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGWSQDLFRA LWRSLSREVK EHVGTDQFGN KYYYIPQYKN WRGQTIREKR IVEAANKKEV DYEAGDIPTE WEAWIRRTRK TPPTMEEILK NEKHREEIKI KSQDFYEKEK LLSKETSEEL LPPPVQTQIK GHASAPYFGK EEPSVAPSST GKTFQPGSWM PRDGKSHNQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NDUFAF2

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