NDUFA4

Alternative Names: Not Available

NDUFA4 is a subunit of mammalian complex I of the mitochondrial respiratory chain. It has both NADH dehydrogenase and oxidoreductase activity, facilitating the transfer of electrons from NADH to the respiratory chain. The enzyme is believed to transfer electrons to ubiquinone.

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  • Sku: PSB-enz-803-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human NADH Dehydrogenase 1 Alpha Subcomplex 4 is a protein belonging to the NADH dehydrogenase (ubiquinone) family. It plays a key role in the mitochondrial respiratory chain, specifically in complex I, where it catalyzes the transfer of electrons from NADH to ubiquinone. This protein has a molecular weight of approximately 51 kDa and is essential for the proper functioning of the electron transport chain. Its recombinant form is commonly used in research settings to study mitochondrial function and dysfunction.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 80% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
11.8 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation NDUFA4 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMLRQIIG QAKKHPSLIP LFVFIGTGAT GATLYLLRLA LFNPDVCWDR NNPEPWNKLG PNDQYKFYSV NVDYSKLKKE RPDF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NDUFA4

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