NANA

Alternative Names: N-acetylneuraminic acid aldolase, N-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, NALase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase, nanA, npl, b3225, JW3194.

N-acetylneuraminate lyase (NanA) is an oxo-acid-lyase enzyme that cleaves carbon-carbon bonds by catalyzing the breakdown of N-acetylneuraminic acid to pyruvate and N-acetyl-D-mannosamine. NanA is classified as a Schiff-base-forming Class I aldolase and is inhibited by reduction with NaBH4 in the presence of substrate, as well as by Cu2+ ions, p-chloromercuribenzoate, and N-bromosuccinimide. In addition, NanA is competitively inhibited by its reaction product pyruvate, as well as structurally related compounds dihydroxyacetone and DL-glyceraldehyde.

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  • Sku: PSB-enz-128-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant E.Coli N-Acetylneuraminate Lyase is an enzyme belonging to the lyase family that catalyzes the reversible aldol cleavage of N-acetylneuraminic acid to pyruvate and N-acetyl-D-mannosamine. This enzyme plays a key role in the sialic acid catabolic pathway. Recombinant E.Coli N-Acetylneuraminate Lyase has a molecular weight of approximately 52 kDa. Its enzymatic activity makes it a valuable tool in various biochemical and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
34.7 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The NANA protein solution (1mg/ml) 20mM Tris-HCl buffer (pH8.0) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPGVGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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NANA

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