mUCHL3

Alternative Names: Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, UCHL3, Ubiquitin Carboxyl-Terminal Esterase L3, Ubiquitin thioesterase L3, Uchl3, Ubiquitin carboxyl-terminal esterase L3.

Ubiquitin carboxyl-terminal hydrolase isozyme L3 is a member of a gene family that breaks down small C-terminal adducts of ubiquitin to form the ubiquitin monomer. It plays a role in regulating neuronal development and spermatogenesis and is linked to neurodegenerative diseases. UCHL3 has a 54% similarity to UCHL1.

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  • Sku: PSB-enz-978-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Mouse Ubiquitin Carboxyl-Terminal Esterase L3 is an enzyme belonging to the ubiquitin carboxyl-terminal hydrolase (UCH) family. With a molecular weight of approximately X kDa, this enzyme plays a significant role in the regulation of ubiquitin-dependent processes. It acts as a deubiquitinating enzyme, catalyzing the removal of ubiquitin moieties from various protein substrates. The Recombinant Mouse Ubiquitin Carboxyl-Terminal Esterase L3 enzyme offers valuable insights into the intricate mechanisms of protein degradation and ubiquitin signaling pathways, making it a valuable tool for scientific research in the field.

Current Lead Time

7-14 Days

Product Specifications

Species
 Mouse
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
 Specific activity is > 9,000 pmol/min/mg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C. Specific activity is > 9,000 pmol/min/mg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 27.2 kDa
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation UCHL3 protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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mUCHL3
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